The crude protein was collected after centrifugation, and purified using the glutathione agarose column then. also created a book poly(lactic-BL21 stress was transfected using the SurR9-C84A-bearing plasmid, and proteins appearance was induced by incubating the bacterias in LuriaCBertani broth mass media filled with 0.01% weight/volume (w/v) ampicillin at 37C. The incubation was terminated after the … Continue reading The crude protein was collected after centrifugation, and purified using the glutathione agarose column then